One year after the events at the Sanctuary, Subaru Natsuki trains hard to better face future challenges. The peaceful days come to an end when Emilia receives an invitation to a meeting in the Watergate City of Priestella from none other than Anastasia Hoshin, one of her rivals in the royal selection. Considering the meeting's significance and the potential dangers Emilia could face, Subaru and his friends accompany her.

However, as Subaru reconnects with old associates and companions in Priestella, new formidable foes emerge. Driven by fanatical motivations and engaging in ruthless methods to achieve their ambitions, the new enemy targets Emilia and threaten the very existence of the city. Rallying his allies, Subaru must give his all once more to stop their nefarious goals from becoming a concrete reality.

Re:Zero kara Hajimeru Isekai Seikatsu 3rd Season is comprised of two arcs. The first eight episodes are subtitled Shuugeki-hen (Attack Arc). The latter eight, subtitled Hangeki-hen (Counterattack Arc), began airing on February 5, 2025. It adapts volumes 16-20 of Tappei Nagatsuki's light novel series of the same title.

The series was released on Blu-ray and DVD in five volumes from January 24, 2025, to June 25, 2025.