Amu Hinamori and the Seiyo Elementary Guardians continue their quest to seek out and purify X Eggs while in pursuit of the elusive Embryo—an egg said to be able to grant any wish. However, the Guardians must secure the Embryo before it falls into the hands of the nefarious Easter Corporation. As part of their plan to lure out the Embryo, Easter hires Lulu de Morcerf, an aloof girl whose ruby necklace gives her the ability to convert Heart Eggs into Mystery Eggs.

These strange Mystery Eggs cannot be purified as easily as X Eggs, and they can force its owner to undergo a Character Transformation. With this growing threat, the Guardians must track down its source and put a stop to them before the matter gets out of hand. Facing insurmountable pressure from these two new objectives, will the Guardians be able to recover the Embryo while also dealing with the Mystery Eggs?

Shugo Chara!! Doki was released by Pony Canyon in 16 compilation DVDs, each containing three to four episodes, from June 17, 2009, to September 15, 2010.

The series was dubbed in Italian by LOGOS srl.