The beautiful Jeanne marries a man named Jean, and the happy newlyweds make their way to the Lord's castle with a cow's worth of money for his blessings. However, the demonic Lord is unmoved by their offering, ignoring their desperate, impoverished pleas. The Lord's wife offers an alternative: Jeanne must become the Lord's conquest for the night in a ritual deflowering.

Scarred by the experience, the shaken Jeanne receives no sympathy from her husband. Instead, she is neglected. But as Jeanne drifts off to sleep, she is met by a strange spirit that encourages her to deliver retribution to those who wronged her. And with a mysterious surge of pleasure and an unquenching libido, Jeanne agrees.

Kanashimi no Belladonna is a captivating, psychosexual adventure that tells a story of cunning witchcraft and deceitful superstition in a poor, rural village of medieval France.

Kanashimi no Belladonna was inspired by the 1862 book La Sorcière (Satanism and Witchcraft) by French historian Jules Michelet. It is the third and final film in the Animerama trilogy conceived by Osamu Tezuka, but it is the only one to be neither written nor directed by him.