The story continues where the first season left off only months into the future. Yuokai Gakuen has been repaired and the new semester is underway. Tsukune and gang are back, and more powerful then ever. Anti-Thesis's true identity is revealed. With new adversaries looming on the horizon, and something much more sinister than the gang has ever faced before.

Tsukune is battling with the monster inside him. With the help of Moka, Mizore, Kurumu, Ruby, and Yukari, Tsukune trains to get his vampire blood under control and the strength to protect what he holds dear.

The new season focuses more on the girls, their past, and their families. New confrontations await them. New friends and allies will join them by their side. Together they will discover the secrets of Moka's rosary, and her lineage...

Rosario to Vampire: Season II was published in English as Rosario+Vampire: Season II by VIZ Media under the Shonen Jump Advanced imprint from April 6, 2010, to May 5, 2015. The publisher later released a complete box set including Rosario to Vampire. It was also published in Italian by GP Manga from September 28, 2013, to September 5, 2015; and in Brazilian Portuguese by Editora JBC from October 2011 to August 2014.